FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_FFLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_FFLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27263280
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT516850.024.88027494-95
CTTCTGC685450.024.45762894-95
TAGACGG27400.023.9357875
TCTTCTG871800.021.42525594-95
TAGAGCG71750.021.3856355
TCTGCTT401500.021.21293894-95
GTCTTCT1027650.019.112694-95
CCTAGAC78800.019.0013243
GTACCGT46900.018.9968686
TATACCG84950.017.916955
GGACTGC55800.017.8888456
ACTGAGC97150.017.6613778
GTCTAGG74250.017.1694811
GACGGTC35800.017.1663257
CTAGACA94050.017.1044584
AGTACCG64550.016.9336575
CACTGAG114050.016.8886787
GTGTATG108400.016.7082251
GACTGTG28150.016.703237
CGTCTTC1192650.016.65519794-95
Produced by FastQC (version 0.11.5)