FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_FFHUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_FFHUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29892905
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG32550.031.050345
CTTCTGC593300.023.08006794-95
TTCTGCT449450.023.04603894-95
GTACCGT54750.021.6246476
GTCTAGG80100.021.0175151
TCTTCTG753350.020.86303394-95
GACGGTC42400.020.6249167
TAGAGCG87500.020.1312665
AGTACCG75850.019.7968965
GACTGTG33950.019.6832797
GTCTTCT883400.019.525494-95
ATAGACG46950.019.330454
TATACCG102200.019.1731745
TCTGCTT372650.018.40252594-95
TACCGTG65650.018.3472237
GGACTGC64950.017.5934856
CGTCTTC1017600.017.4178894-95
AGCGCGC57850.017.1131558
GACAGTG53050.017.1064347
CCTAGAC87400.016.8976573
Produced by FastQC (version 0.11.5)