FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6422600
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGTA2056.55436E-527.35143770-71
AACGTGT2005.9714403E-627.34037862-63
GCGATTA2301.05072104E-725.32111482-83
CTAGGCA2751.6016384E-624.1610472-73
GCTGTAA2052.15834E-723.50442394-95
AACACGC2752.72927E-622.7244762-63
GCCAGTA2704.9377195E-721.56983882-83
CATTCGC1854.1085185E-420.86566484-85
GCTTCAC3155.0152423E-520.7668370-71
CGGTATC2250.003605433320.76682970-71
AACGCGC3056.6957145E-620.48927762-63
CGCTGCC12100.020.41445558-59
GCGCACG6950.020.3439876-77
TCCGAGT1904.94332E-420.30130686-87
TACGGAC1600.00436925320.08983486-87
CCTACGA2508.217325E-620.01031966-67
CGTCCAG2856.1061553E-419.6738470-71
TACGATA5900.019.61312786-87
GCTTACG2307.8884485E-519.58046284-85
GGACGCG3201.0138496E-519.52884362-63
Produced by FastQC (version 0.11.5)