FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_RLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_RLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18708172
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC122750.022.70655358-59
CGCTGCC100650.021.56071558-59
GACGCTG164150.019.78106358-59
TGACGCT185000.017.9877558-59
GCTGCCG79150.017.42889458-59
CATCTGA290100.016.37918358-59
ACATCTG314250.015.9162658-59
CTGACGC223000.015.83595156-57
CACATCT342350.015.58777458-59
TCTGACG236600.015.58210458-59
ATCTGAC259600.015.46012558-59
ACACATC371350.014.78832456-57
CTGCCGA46100.013.82454358-59
TACACAT409300.013.800508556-57
ATACACA453350.012.60654956-57
ATGCGAC27900.011.95535672-73
TATACAC483450.011.9239156-57
GTATTGG50900.011.3402591
TTATACA518550.010.97586654-55
CTTATAC546250.010.65143954-55
Produced by FastQC (version 0.11.5)