FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_RLS-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_RLS-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18812660
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC113250.019.62596958-59
CGCTGCC100900.019.42955258-59
ACATCTG245700.017.73112558-59
GACGCTG141250.016.73504358-59
TGACGCT152000.016.24147858-59
CATCTGA226450.016.08544758-59
CACATCT277200.015.7598858-59
ACACATC304850.015.06126256-57
ATCTGAC201000.014.95126558-59
TCTGACG183100.014.87423356-57
CTGACGC186950.014.17612758-59
TACACAT334400.013.563210556-57
GCTGCCG83000.013.559504558-59
CTGCCGA46950.013.14539158-59
ATACACA375550.012.40330654-55
TATACAC394350.011.60486654-55
TTATACA417000.011.01576756-57
GTATAGG49050.010.5942481
CTTATAC431400.010.59325554-55
GTCTAGG26750.010.2097331
Produced by FastQC (version 0.11.5)