FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_RHW-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_RHW-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13584979
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC101550.024.37724158-59
CGCTGCC81450.023.20378358-59
GACGCTG134050.020.93736558-59
TGACGCT153150.017.98737356-57
GCTGCCG63400.017.580258-59
CATCTGA227050.016.594558-59
ACATCTG247350.016.58768758-59
CTGCCGA36350.016.33115658-59
CTGACGC185300.015.94666656-57
CACATCT267800.015.93927158-59
ATCTGAC212650.015.34440258-59
TCTGACG194800.015.24631756-57
ACACATC294350.015.03259156-57
TACACAT319700.014.20412956-57
TGCCGAC25200.013.94192558-59
ATACACA354200.012.61402254-55
TATACAC379300.012.08569254-55
GTCTAGG21100.011.4857631
GTATTGG35400.011.0204451
TTATACA407350.010.87307654-55
Produced by FastQC (version 0.11.5)