FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_FLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_FLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18029749
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC136450.025.18740858-59
CGCTGCC109150.024.55997358-59
GACGCTG182350.020.75430558-59
TGACGCT213450.019.1321358-59
CTGACGC267650.016.76848458-59
TCTGACG279450.016.37109656-57
GCTGCCG81750.015.97544158-59
CATCTGA324950.015.65316758-59
ATCTGAC302050.015.16044556-57
CACATCT359100.014.89423556-57
ACATCTG343600.014.87414458-59
ACACATC384750.014.6599958-59
TACACAT414350.013.75593756-57
CTGCCGA54750.013.44080858-59
ATACACA459150.012.63433356-57
TATACAC495350.011.62401454-55
CGTACGA22800.011.1053770-71
TTATACA541900.010.75672254-55
TGCCGAC40650.010.24506758-59
CCTAGAC26050.09.9836513
Produced by FastQC (version 0.11.5)