FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_FHW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_FHW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21859952
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC251500.031.31634358-59
ACGCTGC360050.028.56671558-59
GCTGCCG169050.026.32521458-59
GACGCTG490000.022.90796958-59
CTGCCGA105750.022.47745558-59
TGACGCT596300.019.54408858-59
TGCCGAC76300.018.7343958-59
TGACGCC36750.017.6299958-59
CTGACGC710950.017.38627256-57
TCTGACG753750.016.69161656-57
ATCTGAC790300.015.78013256-57
CATCTGA821800.015.38837854-55
CACATCT868150.015.25168356-57
ACATCTG849550.015.10714256-57
GACGCTT25950.014.98039558-59
ACACATC900700.014.67170456-57
TACACAT961850.013.89179954-55
GAGTGTA38700.013.07941958-59
ATACACA1066700.012.48375654-55
GCCGACG84400.011.61080558-59
Produced by FastQC (version 0.11.5)