FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_FFS-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_FFS-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18783753
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC107950.022.28054258-59
CGCTGCC87450.022.0673358-59
GACGCTG138900.019.57832758-59
TGACGCT159850.019.23029558-59
CATCTGA255200.016.67083458-59
CTGACGC201350.016.3872658-59
TCTGACG208150.016.24358756-57
ACATCTG272700.015.86694158-59
ACACATC322850.015.5610858-59
CACATCT296300.015.33739158-59
ATCTGAC231600.014.75656356-57
TACACAT356050.014.25025156-57
GCTGCCG70500.013.54357458-59
ATACACA396800.013.28484556-57
TGCCGAC31100.012.56566258-59
CTGCCGA49350.012.4088258-59
TATACAC429500.012.0083356-57
AGTGCGC23550.011.21607162-63
TTATACA472350.011.16909754-55
ACGCTAC25400.011.01086762-63
Produced by FastQC (version 0.11.5)