FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_FFLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_FFLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19602933
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC75350.026.0528858-59
ACGCTGC100950.024.23775758-59
GACGCTG141650.022.05439658-59
TGACGCT169000.020.29798358-59
CTGACGC216450.016.70493958-59
ACATCTG293450.016.48379558-59
CACATCT319250.016.47743658-59
TCTGACG226500.016.46340856-57
CATCTGA276000.016.05087358-59
ACACATC343450.015.7506758-59
ATCTGAC252000.014.94019958-59
TACACAT385100.014.64381658-59
GCTGCCG62150.014.52837258-59
TACCGAC9001.1732482E-913.7144676-77
ATACACA436850.013.37933156-57
ACGACTA25700.012.89380562-63
TATACAC472150.012.70204456-57
GTCTAGG31150.012.0148921
CCTAGAC31050.011.8624953
TAAGGTG30350.011.747745
Produced by FastQC (version 0.11.5)