FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n02_FFHW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n02_FFHW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18760965
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC85150.025.56019258-59
ACGCTGC118500.025.1691458-59
GACGCTG165850.022.43061658-59
GCTGCCG66200.020.82203358-59
TGACGCT196600.019.33228558-59
CTGACGC245400.016.6375758-59
ACATCTG327900.016.34802258-59
TCTGACG262250.015.92169556-57
ACACATC376800.015.54210158-59
CATCTGA307650.015.52445858-59
CACATCT352100.015.33888458-59
CTGCCGA49800.015.05353958-59
ATCTGAC290250.014.94152958-59
TACACAT417350.014.15789656-57
GTCTAGG31100.013.4536811
ATACACA475650.013.0096156-57
CCAGGGT13300.012.7377713
TATACAC514400.012.4554956-57
GCTGTCC15100.012.27823458-59
TGCCGAC34750.012.1783858-59
Produced by FastQC (version 0.11.5)