FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6422600
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA2456.184564E-1122.16116394-95
CACTGAG5400.021.359267
ACCGCGT3153.8198777E-1119.716248
ACTACAC4600.019.2919793
ACCGTGC4800.018.4839748
TTAGGAC4150.018.17633
GGACCGT3251.2132659E-917.7486256
TACACGG2253.2024964E-617.7486235
ACCGTGA3802.910383E-1117.5111358
TACCGTG3301.4915713E-917.4757587
GTAACAC2354.84709E-616.9933623
GTAGGAG6050.016.8685263
ACACCGC4259.094947E-1216.7045866
ACAGTGC4259.094947E-1216.7008158
AGTACCG2951.381868E-716.5453285
GTGTACA5950.016.4106351
ACTGCGC3252.3355824E-816.3796468
CTATACT6000.016.269574
GTGTAAG2751.2027594E-616.1393851
GCCTAAG4401.6370905E-1116.1393851
Produced by FastQC (version 0.11.5)