FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_RLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_RLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18708172
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGC355150.025.68903494-95
TTCTGCT282450.025.64232494-95
TCTTCTG447450.022.89715894-95
TCTGCTT229250.022.4892694-95
GTCTTCT504450.021.0948394-95
TAGACGG26700.019.617045
CGTCTTC594500.018.43937992-93
TATACCG54050.018.3872245
CCGTCTT655700.018.27323394-95
GCCGTCT766800.017.12637394-95
TACACCG50000.017.0114965
CTACACT52550.016.7823354
GTCTAGG58250.016.7579331
TGCCGTC914700.016.53058694-95
AGTACCG40700.016.4989285
CCTAGAC57600.016.3213353
CTGCTTG163100.016.10867994-95
ATGCCGT1015100.015.87482994-95
TATACTG86000.015.5643735
GGACTGC51000.015.5368156
Produced by FastQC (version 0.11.5)