FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_RLS-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_RLS-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18812660
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT255700.023.86161694-95
CTTCTGC315350.022.86947694-95
TCTGCTT208550.019.93312694-95
TCTTCTG389450.019.39793694-95
GTCTTCT428200.018.67407494-95
TACACCG49900.017.5819345
GTATAGG85400.017.5633661
ATGCCGT800850.016.53633794-95
TATACCG57150.016.5263655
CCGTCTT521750.016.4030992-93
CGTCTTC507850.016.33200592-93
GTCTAGG50700.016.072531
TGCCGTC730700.016.0448494-95
TAGACGG27200.015.962985
GCCGTCT609950.015.82364294-95
CTATACT89600.015.6367854
TAGAGCG44500.015.3901395
TATACTG77600.015.0553165
CTGCTTG158000.014.99722794-95
CCTAGAC52950.014.8784543
Produced by FastQC (version 0.11.5)