FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_RHW-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_RHW-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13584979
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT220100.027.74798294-95
CTTCTGC287300.026.18865894-95
TCTGCTT174350.024.84872694-95
TCTTCTG358300.023.41286394-95
GTCTTCT410300.021.05786194-95
TAGACGG16600.019.6894115
CTGCTTG126800.018.86979794-95
CGTCTTC477800.018.7974792-93
GTCTAGG43750.018.5276721
GTGTAGG41500.017.9134941
CCGTCTT532900.017.47290894-95
TACACCG31350.017.2808785
GCCGTCT618500.016.83912394-95
TGCCGTC741350.016.52068394-95
AGTACCG31850.016.447295
CCGTCTA20150.016.35028394-95
GTACCGT25900.016.0768576
ATGCCGT826450.016.00051794-95
TACACAG40950.015.8537525
GTGTATG60500.015.84085751
Produced by FastQC (version 0.11.5)