FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_FLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_FLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18029749
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT299200.029.23643194-95
CTTCTGC399950.027.90583694-95
TCTTCTG502600.024.48440694-95
TCTGCTT237050.024.34050894-95
GTCTTCT586450.022.64099394-95
TAGACGG24250.020.3083425
CGTCTTC697550.019.62533694-95
CCGTCTT777900.018.5322492-93
CTGCTTG178300.016.93105794-95
GCCGTCT891400.016.3636392-93
CTAGACA73150.015.6682034
CCTAGAC53800.015.6447673
GACTGTG31000.015.4506157
TACACCG43300.015.4060725
TAGAGCG48650.015.3683475
TATACCG55600.015.2993275
AGTACCG44250.015.2776735
CCGTCTA29150.015.11738594-95
GTCTAGG55500.015.0886151
TATACTG81000.014.97882655
Produced by FastQC (version 0.11.5)