FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_FHW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_FHW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21859952
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT722400.034.788994-95
TCTGCTT521950.032.9483694-95
CTTCTGC998200.032.38957294-95
CCGTCTA37500.030.52209794-95
CTGCTTG345550.027.54346394-95
TCTTCTG1297500.027.19146294-95
TGCCGTA55850.023.28506994-95
GTCTTCT1546750.023.24203394-95
GTATGCG36000.022.22150894-95
TAGACGG22250.021.5320155
ATGCCGG57150.021.10437894-95
CGTCTTC1845200.020.66405992-93
TGCTTGA260900.019.73381694-95
CGTCTTA64500.019.46270494-95
GTCTAGG53600.018.5485731
CCGTCTT2120650.018.37204792-93
TCGTATA62550.018.36420694-95
TATACCG54550.018.221715
AGTACCG42000.017.6965945
ATGCCGA65500.017.66238294-95
Produced by FastQC (version 0.11.5)