FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_FFS-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_FFS-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18783753
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT245850.024.89281594-95
CTTCTGC313600.024.52860894-95
TCTTCTG385750.022.74366294-95
TAGACGG22550.022.4297735
GTCTTCT445050.021.41934894-95
TCTGCTT206300.019.44330694-95
CGTCTTC520800.019.23892494-95
GTACCGT37000.018.3880166
CCGTCTT570150.018.23405892-93
GTCTAGG57150.017.7046811
GCCGTCT659700.017.10232294-95
TGCCGTC786550.017.01992294-95
ACTGAGC77400.016.9408518
AGTACCG48500.016.5197815
GACGGTC32450.016.273317
TACACCG45000.016.2131755
ATGCCGT877850.016.07244194-95
CCTAGAC55600.016.0203233
CACTGAG84500.015.9940637
TATACCG61400.015.9649925
Produced by FastQC (version 0.11.5)