FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_FFLUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_FFLUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19602933
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT252950.027.27193894-95
CTTCTGC323300.026.6368594-95
TAGACGG24600.024.928575
TCTTCTG411200.024.05514594-95
GTCTTCT477550.023.1162294-95
GTCTAGG60650.020.8172971
TAGAGCG56200.020.469575
TATACCG67450.020.3072625
CGTCTTC546750.020.05457994-95
CCGTCTT623550.018.9997192-93
AGTACCG52000.018.7657135
TCTGCTT218800.018.60389394-95
CCTAGAC60850.018.5375293
CTAGACA71900.017.9298024
GTGTATG85200.017.39381
GCCGTCT708200.017.33641694-95
GTACCGT37900.017.2435056
TGCCGTC839500.016.63625594-95
CACTGAG86500.016.5564887
ATAGACG32900.016.4627114
Produced by FastQC (version 0.11.5)