FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l01_n01_FFHW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l01_n01_FFHW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18760965
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG20300.028.2229585
CTTCTGC366150.027.32720294-95
TTCTGCT279300.027.1605894-95
TCTTCTG471300.024.98046794-95
GTCTTCT540800.022.25884694-95
TCTGCTT241450.021.75508194-95
GTCTAGG55900.021.4643081
TAGAGCG54850.020.6458365
CGTCTTC619800.020.42154192-93
GTACCGT31300.019.8773756
TATACCG63200.019.830315
CCTAGAC58750.019.1991163
CCGTCTA25150.019.04674594-95
GACTGTG18750.018.8554977
GACGGTC24350.018.7462067
CCGTCTT717400.018.48330994-95
ACTGAGC70300.018.3973268
GTCTATG62900.018.3638171
AGTACCG45950.018.118245
CACTGAG80450.017.967547
Produced by FastQC (version 0.11.5)