FastQCFastQC Report
Mon 10 Oct 2022
H37NLDSX5_n01_f2HRB0244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH37NLDSX5_n01_f2HRB0244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCCGGCTCAGCTGCGCCATAGATCGGAAGAGCACACGTCTGAACTCCA50.4668534080298786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGCCTCGGACCGCGACCCCAGGTCAGGCGGGAAGATCGGAAGAGCACA40.3734827264239029No Hit
GTTGGTAAAACATGGTGCCTGCTTGGTTGCCTGTTTTGTCGACTGTTGGG30.2801120448179272No Hit
CCATCCAAGCAAAGGGAGCGCTGGAGCAGGATCGGAAGATCGGAAGAGCA30.2801120448179272No Hit
GCAGCCGAGGCGGCTGGTCTAGATTAGTGAGAAAGATCGGAAGAGCACAC30.2801120448179272No Hit
CCTTGTTCCCGTTCCAGTTGCTCATGCTCCAAGCGAGATCGGAAGAGCAC30.2801120448179272No Hit
CGAAACCAACCTGCTGAAGCTGAAAAATACACTGTTGTGCAAGATCGGAA30.2801120448179272No Hit
CACTCTGTTCAGTATGGGATGAGCAAACCTGAAGCTGACAGCCATGAGGC30.2801120448179272No Hit
CCGACCATCTCATCCGCGGCGCCGCCATTGCCAGATCGGAAGAGCACACG30.2801120448179272No Hit
CGTCTTGTCCGGTCGGAGTATGTGCAATGTATCAGTATTTAGAAAGATCG30.2801120448179272No Hit
CATACGACAACAATTTCAAAACGAGATGCGAAGATACTCAGAACTCCCAT30.2801120448179272No Hit
CGTCGCAGGCACTGCATTACTCGTTAAGATCGGAAGAGCACACGTCTGAA30.2801120448179272Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTTGGAAGTTGACGACGAACTGCTGCCACAGGTCTGCCCACGAAGAA30.2801120448179272No Hit
GGGGTCAGATCACAGAGACCAGAGGCATTTCAGATCGGAAGAGCACACGT30.2801120448179272No Hit
ATGCTTATTTTGTTTGTGTTATATTCGCAAGGATCTATACAAGATCGGAA30.2801120448179272No Hit
GCCAGATCTCTACCCGCGACAATGTCTTCTTCCGCAGATCGGAAGAGCAC30.2801120448179272No Hit
CGCGCGCCACGTCGCCGGCGTAATGGCGCCAGATCGGAAGAGCACACGTC20.18674136321195145No Hit
AGCGGCGCACTGCAACCGAGCGGCGCACCTCCGCAGACAAGATCGGAAGA20.18674136321195145No Hit
ACTAAACAATGCCTATATGTTACTCCAACATCAGCTCTTTTGTACAAGAT20.18674136321195145No Hit
GCGGTGGATCGTGCGGCGGAAATAGATCGGAAGAGCACACGTCTGAACTC20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGCGCGCGGGGAGAGGAGAGAGAGAGAGCGGGGGGAGGAGAGATGGGC20.18674136321195145No Hit
GCAATGCCTTACAAGTTGGCTATACACTCTGGAGATCGGAAGAGCACACG20.18674136321195145No Hit
CTGTTAAGCTGAACCTTCAGGTGTCTAAGAGATCGGAAGAGCACACGTCT20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTCATTTTCTCGTCGTCCCAAACTCTCTAGAGAGCAAGTCAAAAGATC20.18674136321195145No Hit
CTCAAAGCAAGCCATCGCTCTGGATACAAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAGGCTAGGCGGCTGGGTGGCCACCAGCCGGCGTCTAGCTCGGGCACA20.18674136321195145No Hit
GACACATCTGAGTCGAACAAGAACAAAAATCGCAAAAACGGAGATCGGAA20.18674136321195145No Hit
CCAAATCCACCCACCGCTGATGACTGGAAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTTATCCTACATGGTGGCTGAGTCAGCGTGGGACTCACGTGAGCCCTA20.18674136321195145No Hit
ACTAGTATTAAGGAACACCGCATAATAAGCTTTCTTTCTCGAACTATTTT20.18674136321195145No Hit
GTTCTAAATTTCAAGTAATAAGGATTCATTTGTTTAAGCACAAAAGAGAT20.18674136321195145No Hit
CATGCTCCTATGTGGTCCTACTATCCTCACCATTAAACTTGGTAAATTCG20.18674136321195145No Hit
TTCCCGTGGACCCGGTCCGCGCGCCCTCTCGCCCGGAGATCGGAAGAGCA20.18674136321195145No Hit
AGATTACACGCGGTGGACAAGAATGACCGATTTGTGAAGGTCGGAAGAGC20.18674136321195145No Hit
CATTTTTTCGATTTAACGTGCAGCCAGTTAATTCCGAGATCGGAAGAGCA20.18674136321195145No Hit
AAATAATACAAAACCATTCGATATCACATAGCCTCACCATCAGAGCCACA20.18674136321195145No Hit
CTTCTCCTCGCCTTGTAGGGAGAGAGAGAGAGGAGATAGAGAGAAGAGGG20.18674136321195145No Hit
CTTCACCCCCTAAAGTATTGGGCCTGAAAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCACCAAAGGCAAAGAAGGCCCCAATTCCCAAAGATCGGAAGAGCAC20.18674136321195145No Hit
TTGGGTAGATTAAGCCTAACCTGTTAAAATCCGCGAGCCCCTTCTCATAT20.18674136321195145No Hit
CATCGACTCGGCGAATATCAGATCGGAAGAGCACACGTGTGAACTCCAGT20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGTGTTGTTTTGTATATTATTACACCCAGACTTTCGGGGGGGAAGGGAA20.18674136321195145No Hit
GGAGCTTCTAGGACAAGGAATATATGCTAGGTGGGCAGATCGGAAGAGCA20.18674136321195145No Hit
TCCTATACCATATAACGATACAAATAAACATATTGAAATTAAAACAAAGA20.18674136321195145No Hit
CCCGGCGCGCCGCACCCGAACCACGCGCACCTGAAACCCAGATCGGAAGA20.18674136321195145No Hit
ATGGTCTGGTGAATCGAGGCCAAAGATGATAGTGCCAAAGAAGCAGATCG20.18674136321195145No Hit
TGTCTTAACTGCCTCTTTTCACTTGTCTTAGATGCCGCGGTACAAGCCTG20.18674136321195145No Hit
ATCTCCCCAGGCACCAGCCCCGACCAGAACCACTTGAGGACTGGGACTTG20.18674136321195145No Hit
TTCAAACAAAGAAACCGAAAAGCAAGGAGAAGATCGGAAGAGCACACGTC20.18674136321195145No Hit
TGTACGCCGCTGCCCTGATATCTTCAACCATCGGAGATCGGAAGAGCACA20.18674136321195145No Hit
ATGGTATATCCGTGCGCTTGCGGATTACTTCTGTAAAGATCGGAAGAGCA20.18674136321195145No Hit
CGCCCGCCCCTCGGCTGACGCAATAATAGATCGGAAGAGCACACGTCTGA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCTGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCAAGATCGGAAG20.18674136321195145No Hit
CCGCCCTCTCCACCGCCATCTCCGCCCTCTCAGATCGGAAGAGCACACGT20.18674136321195145No Hit
TGTTTAATGCAAGCTTGGAAGAGGGGCAAGGTTGTTTTGCAAATCTAGAT20.18674136321195145No Hit
GATGAGACTAAACTTGCTCTTCCGGAGCAATGAGAGATCGGAAGAGCACA20.18674136321195145No Hit
TAGCTCAGTGCAACAAATTCAAGCGAGCCTCTAGATCGGGAAGAGCACAC20.18674136321195145No Hit
CGAGCGATGGGCGGCGGCAGGTGGTGACGGTGCCCTCTAGATCGGAAGAG20.18674136321195145No Hit
CTCTTTCCTTCACTCCGCACCTTTGGGCGCGCCACTTTCGGAGAGATCGG20.18674136321195145No Hit
GAGCATTGTTTGGGTTTGTTTTTTTGTTTAATGTAGGTTTCGCCATCGGT20.18674136321195145No Hit
GAAAACTGTGCTAGGCATGTTTGATAGTACCTCACCAGATGGTTGATGCA20.18674136321195145No Hit
CCTCCTCCTCTCATGCTAGATCAAAGATGTCAGGTTTAGATCGGAAGAGC20.18674136321195145No Hit
CGTGCTCTTAATGATCTAAAAGCAAAGGCTGAAAAATAAACAGATCGGAA20.18674136321195145No Hit
GAAGACCATGCCCTCATTTCAGAAGCTAAGTTCACAGATCGGAAGAGCAC20.18674136321195145No Hit
CTTGAAACACTGCGAGGCCGCTTTCTACCCTGTGTAAAGATCGGAAGAGC20.18674136321195145No Hit
ATTGGGCAATTGAAAGATAAAAAGTACTTACATGTCTTTCAGCCTGCTCC20.18674136321195145No Hit
CGTTTGAGTGGCGACTTCAATTTAGAAGAGGCTGAAAGGGTTTGCAAAGT20.18674136321195145No Hit
CAAATAAGCCAGCTAGCTGGTAGTTAATCAGGAGATCGGAAGAGCACACG20.18674136321195145No Hit
GCTGATATTTCCCTTTGTTCGACTTCGGTTCAAAAGATCGGAAGAGCACA20.18674136321195145No Hit
TGGATTATTCCATGAATTCCTCAAAACGAGGCTCATCAAAATGCAAAATC20.18674136321195145No Hit
ATAAAGAGACTTCTTTTGCTGTGATATTAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTAGAAATTATTTTAAAAGCCTACAAGCTAAACCCTAATTTTAGATC20.18674136321195145No Hit
CACCCCATAGCTATATATGTCACCATCCATGGAGACCTGAGATCCGGAGC20.18674136321195145No Hit
TCACCAGCTTCGCAAGAGAAGACGTCAGCGAGAACTCATCGGGATTCGTC20.18674136321195145No Hit
AACATTTTTTGTGGAACAAAAAATAAACCGAATTCCAGATCGGAAGAGCA20.18674136321195145No Hit
GGTAAGTTCCGACCCGCACGAAAGGCGAGATCGGAAGAGCACACGTCTGA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTTTGAAAAGCATGCACGTGGAAAACGAGTGAGGTGAAGATCGGAAGA20.18674136321195145No Hit
AGTGCAATTTTAAATTCCAGGGAAATTCCTTTTTCTAGATCGGAAGAGCA20.18674136321195145No Hit
GTGATGAAGTCGAGGCTGCCGAACCAGAAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGAAAGGGGAGCAGAAGCCGGCCGGCGAGAAGGCCGAGATCGGAAGA20.18674136321195145No Hit
CCGTCGGCGAAGCATGAGCTGAACCGCGCAGATCGGAAGAGCACACGTCT20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGGATCGACCGAACCAGCTATTGAAGCGGAAAAGAACAAAAAGATCG20.18674136321195145No Hit
TATTTAGTTTGTTTTTTCTTAGAATTTGCAGGATTTTCTCTAATTAGATC20.18674136321195145No Hit
CAAAAACCTGCCAAAATTTGATAACAAGATCGGAAGAGCACACGTCTGAA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCTTCCCTTTTTGTTTAAGGCAGTGCCTCGTATTCATAAGATCGGAAG20.18674136321195145No Hit
GTAAACGCTACGGCCTTGTCATTGCGCGCCACGCTAAGAGACCGGAAGGT20.18674136321195145No Hit
TCCAAGTCTGCTGCAATTGGTAATAGGAGATAAGCAATTGGTGGTCGTAG20.18674136321195145No Hit
TCTTGAAACTGGTGAACCAGATTGTCGAATAGATGGCCTAGTATAGATCG20.18674136321195145No Hit
GCTCGTAGAGGAGGAAAAGTTTGAGTAAGATCGGAAGAGCACACGTCTGA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCTCTACGAAACTACCAAAGTCCTACTCGAGATCGGAAGAGCACACG20.18674136321195145No Hit
CGTTAATACAAATTCACTAACATAACCTATCACCGAAAGGCGCATATCAC20.18674136321195145No Hit
GCCCACAGCATAGTACTCCGATCGAACGTGGGAGAGAGATCGGAAGAGCA20.18674136321195145No Hit
GTGGTACCCGGCACGTGGCAAAGATCGGAAGAGCACACGTCTGAACTCCA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTTTGGTACCTCCTCAAGGACCGTAGAATTGCTCAAAAAATATATTAG20.18674136321195145No Hit
TGGTGCAGCTTGAGGTCCCAAAACAATGCGCGGCCTCTGGCTACTAAGAT20.18674136321195145No Hit
TCTCCAAGTTTTCCCCTTGTTCTGCTGGTTGATTCGACTCTTGCGTATAG20.18674136321195145No Hit
TTCTACTTTGTCTCTTTCCAGGGCTTCAATTTGTAGATCGGAAGAGACAC20.18674136321195145No Hit
CTGCTGCTAGTTGTTATTTACAAATTCTTTGGCTTTTATCTACTTTGAAA20.18674136321195145No Hit
AGAAAAAAATACAATAATTATAGCAAGAGTTTTTCTTAGCATGACCAGAT20.18674136321195145No Hit
TGTTTGATAAAAAAAGAGTGGTGCACGATAAGTGGCTAAGGAAACCAATA20.18674136321195145No Hit
TATAATTTAACAAAGCCAGCATCACTGACCAAAAGTTGATAATAGATCGG20.18674136321195145No Hit
AACTAGAACTATTTTGACTTTGACAAAAGTCGAAATGACTTAAAGGTCAT20.18674136321195145No Hit
CGTTCCCCCCAAATCTCACCGGACAAGATCGGAAGAGCACACGTCTGAAC20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGTGTTTTGCTTTCGTGGAACAAGTAAGATCGGAAGAGCACACGTCTG20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTCATAACTAATTCATCCGAGCTCCGTGTAAGCCCATTCAAGTCTCAG20.18674136321195145No Hit
TTACCCCGTCCCTTAGGATCGGCTTACCCATGTGCAAGTGCCGAGATCGG20.18674136321195145No Hit
CCCTCTGAAAAATCTAGTCATTGTGTTCTCATTAGATTCTTGTAAATCAC20.18674136321195145No Hit
CCAAAATTTATGGCTCAGTATAACAAACTTCTGAGATCGGAAGAGCACAC20.18674136321195145No Hit
AAAATGGAAGTGATCAAAATAGTTTTAGATCGGAAGAGCACACGTCTGAA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCGCCGCCGGCCATGGCCTCCTCCTAGCTCGCTGCTCTCGTCTAGATC20.18674136321195145No Hit
ACGATCGCTTTTTGGAGTTTTGGTGATAGATCGGAAGAGCACACGTCTGA20.18674136321195145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTGTTCACACAGCCGAAGAAGTCAACCAGCAATGGGAATCAAGGGCTC20.18674136321195145No Hit
GATGGAAAGGTGAATTTCCAATCCTACCTACCCCCACTTGTGGGATCAGT20.18674136321195145No Hit
TTTGAAGCGGCCAGCTCTTAGCGCAGAACCATCGATTCAAGTAGATCGGG20.18674136321195145No Hit
GTCACCCCCAGAAGCACCCTGCCTTTGTGTATTTCTATGGTAAGAAGATC20.18674136321195145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)