FastQCFastQC Report
Fri 11 Nov 2022
H37NLDSX5_n02_f2HRB0244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH37NLDSX5_n02_f2HRB0244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGCAGCTGAGCCGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGA50.4668534080298786Illumina Single End PCR Primer 1 (100% over 29bp)
TCCCGCCTGACCTGGGGTCGCGGTCCGAGGCGTTAGATCGGAAGAGCGTC40.3734827264239029No Hit
CTGACTGTGACCGTCTCCGACCGAGTGCTGCCTCATGGCTGTCAGCTTCA30.2801120448179272No Hit
TTCTCACTAATCTAGACCAGCCGCCTCGGCTGCAGATCGGAAGAGCGTCG30.2801120448179272No Hit
GATATTCGCCGAGTCGATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT30.2801120448179272Illumina Single End PCR Primer 1 (100% over 31bp)
CAGAAGTTTGTTATACTGAGCCATAAATTTTGGAGATCGGAAGAGCGTCG30.2801120448179272No Hit
CCGATGGTTGAAGATATCAGGGCAGCGGCGTACAAGATCGGAAGAGCGTC30.2801120448179272No Hit
CCCCTGATCCAACGTTCATCTCCAGAATAAACTATCTAGCCGAGCCCAAC30.2801120448179272No Hit
CGCTTGGAGCATGAGCAACTGGAACGGGAACAAGGAGATCGGAAGAGCGT30.2801120448179272No Hit
TTCTTCGTGGGCAGACCTGTGGCAGCAGTTCGTCGTCAACTTCCAAGGAA30.2801120448179272No Hit
TGTATAGATCCTTGCGAATATAACACAAACAAAATAAGCATAGATCGGAA30.2801120448179272No Hit
TTCTAAATACTGATACATTGCACATACTCCGACCGGACAAGACGAGATCG30.2801120448179272No Hit
TCCGATCCTGCTCCAGCGCTCCCTTTGCTTGGATGGAGATCGGAAGAGCG30.2801120448179272No Hit
GAAATGCCTCTGGTCTCTGTGATCTGACCCCAGATCGGAAGAGCGTCGTG30.2801120448179272No Hit
GGCAATGGCGGCGCCGCGGATGAGATGGTCGGAGATCGGAAGAGCGTCGT30.2801120448179272No Hit
TAACGAGTAATGCAGTGCCTGCGACGAGATCGGAAGAGCGTCGTGTAGGG30.2801120448179272Illumina Single End PCR Primer 1 (100% over 24bp)
GCGGAAGAAGACATTGTCGCGGGTAGAGATCTGGCAGATCGGAAGAGCGT30.2801120448179272No Hit
CACTAGCATGGGAGTTCTGAGTATCTTCGCATCTCGTTTTGAAATTGTTG30.2801120448179272No Hit
AGTTCTCTGTTGTACATAAAAAATAGTTCGAGAAAGAAAGCTTATTATGC20.18674136321195145No Hit
CGAGTAGGACTTTGGTAGTTTCGTAGAGATATAGATCGGAAGAGCGTCGT20.18674136321195145No Hit
ATACTAGGCCATCTATTCGACAATCTGGTTCACCAGTTTCAAGAAGATCG20.18674136321195145No Hit
AAGTGCATCAACCATCTGGTGAGGTACTATCAAACATGCCTAGCACAGTT20.18674136321195145No Hit
AATATCACAGCAAAAGAAGTCTCTTTATAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
GCAACTTTGCAAACCCTTTCAGCCTCTTCTAAATTGAAGTCGCCACTCAA20.18674136321195145No Hit
TCAGCATTGATCTCTTTTGTGCTTAAACAAATGAATCCTTATTACTTGAA20.18674136321195145No Hit
GGAATTCGGTTTATTTTTTGTTCCACAAAAAATGTTAGATCGGAAGAGCG20.18674136321195145No Hit
TGTCTGCGGAGGTGCGCCGCTCGGTTGCAGTGCGCCGCTAGATCGGAAGA20.18674136321195145No Hit
GCTTCTTTGGCACTATCATCTTTGGCCTCGATTCACCAGACCATAGATCG20.18674136321195145No Hit
CTAAAACTGATCCCACAAGTGGGGGTAGGTAGGATTGGAAATTCACCTTT20.18674136321195145No Hit
TGGCCTTGCTGCCACGATCCACTGAGATCCAGCCCAGCGTAGATCGGAAG20.18674136321195145No Hit
TACTCAAACTTTTCCTCCTCTACGAGCAGATCGGAAGAGCGTCGTGTAGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 23bp)
AAACCTGACATCTTTGATCTAGCATGAGAGGAGGAGGAGATCGGAAGAGC20.18674136321195145No Hit
TGTACAAAAGAGCTGATGTTGGAGTAACATATAGGCATTGTTTAGTAGAT20.18674136321195145No Hit
ATCACCAAAACTCCAAAAAGCGATCGTAGATCGGAAGAGCGTCGTGTAGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 23bp)
TGTATCCAGAGCGATGGCTTGCTTTGAGAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
CTCCTCTTTTCCTTCTCCTGCTTCTTCTNAGGGGAGTTGAGCTTGCCGAA20.18674136321195145No Hit
AGAGGGCACCGTCACCACCTGCCGCCGCCCATCGCTCGAGATCGGAAGAG20.18674136321195145No Hit
TCCCGACGAATCCCGATGAGTTCTCGCTGACGTCTTCTCTTGCGAAGCTG20.18674136321195145No Hit
CTCCGAAAGTGGCGCGCCCAAAGGTGCGGAGTGAAGGAAAGAGAGATCGG20.18674136321195145No Hit
ATTATCAACTTTTGGTCAGTGATGCTGGCTTTGTTAAATTATAAGATCGG20.18674136321195145No Hit
AGCCTCCCTCTTCTCTCTATCTCCTCTCTCTCTCTCCCTACAAGGCGAGG20.18674136321195145No Hit
ATTTCTTTGTTTTAATTTCAATATGTTTATTTGTATCGTTATATGGTATA20.18674136321195145No Hit
GGTCATGCTAAGAAAAACTCTTGCTATAATTATTGTATTTTTTTCTAGAT20.18674136321195145No Hit
GGCGCCATTACGCCGGCGACGTGGCGCGCGAGATCGGAAGAGCGTCGTGT20.18674136321195145No Hit
TCGGATTGGATGATTTTCGTGCTCAGAGATCGGAAGAGCGTCGTGTAGGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 24bp)
CCCGGCCCATCTCTCCTCCCCCCGCTCTCTCTCTCTCCTCTCCCCGCGCG20.18674136321195145No Hit
TTACAGAAGTAATCCGCAAGCGCACGGATATACCATAGATCGGAAGAGCG20.18674136321195145No Hit
TCCCTTCCCCCCCGAAAGTCTGGGTGTAATAATATACAAAACAACACTGA20.18674136321195145No Hit
ACATTCCTCAAGTTTCATATTTAGAGAATGAAGTCTTAACTAAGGAATTT20.18674136321195145No Hit
TAGTAGCCAGAGGCCGCGCATTGTTTTGGGACCTCAAGCTGCACCAAGAT20.18674136321195145No Hit
TATGAATACGAGGCACTGCCTTAAACAAAAAGGGAAGGTGAGATCGGAAG20.18674136321195145No Hit
GTGAACTTAGCTTCTGAAATGAGGGCATGGTCTTCAGATCGGAAGAGCGT20.18674136321195145No Hit
ATACGCTCCTCCTCCTCCTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 30bp)
TTACACAGGGTAGAAAGCGGCCTCGCAGTGTTTCAAGAGATCGGAAGAGC20.18674136321195145No Hit
CCGGAGATGGTTTAGCCGGCCCTCATCAACAGCCTGCAATTGCTCAAATA20.18674136321195145No Hit
TCTGGTTCGGCAGCCTCGACTTCATCACAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
TAATCTGGATTGTGCAGAGTGTTGAGAAGTTTTGTGGCTCTGATGGTGAG20.18674136321195145No Hit
CTGAGACTTGAATGGGCTTACACGGAGCTCGGATGAATTAGTTATGAATT20.18674136321195145No Hit
AAAATTAGGGTTTAGCTTGTAGGCTTTTAAAATAATTTCTAAAAAAGATC20.18674136321195145No Hit
ACTTGAATCGATGGTTCTGCGCTAAGAGCTGGCCGCTTCAAAAGATCGGA20.18674136321195145No Hit
CTTAGACACCTGAAGGTTCAGCTTAACAGAGATCGGAAGAGCGTCGTGGT20.18674136321195145No Hit
CCGTTTTTGCGATTTTTGTTCTTGTTCGACTCAGATGTGTCAGATCGGAA20.18674136321195145No Hit
CGGCACTTGCACATGGGTAAGCCGATCCTAAGGGACGGGGTAAAGATCGG20.18674136321195145No Hit
TTGCCACGTGCCGGGTACCACAGATCGGAAGAGCGTCGTGTAGGGAAAGA20.18674136321195145Illumina Single End PCR Primer 1 (100% over 29bp)
TTGTTTTGTTCTATGAATTCAATGTCATGTTAGCAATCTGATATTGGTTT20.18674136321195145No Hit
CTTCTCGCGGAGCAGAGGTGGTGGCGGATAGATCGGAAGAGCGTCGTGTA20.18674136321195145Illumina Single End PCR Primer 1 (100% over 21bp)
AGAGGCTCGCTTGAATTTGTTGCACTGAGCTAAGATCGGAAGAGCGTCGT20.18674136321195145No Hit
TCTCCTTGCTTTTCGGTTTCTTTGTTTGAAAGATCGGAAGAGCGTCGTGT20.18674136321195145No Hit
GCGCGGTTCAGCTCATGCTTCGCCGACGGAGATCGGAAGAGTGTCGTGTA20.18674136321195145Illumina Single End PCR Primer 1 (95% over 21bp)
GCTTTTCGAAAGTTTGATATCCTTCATAGTTTTCTTGGCAGAGATCGGAA20.18674136321195145No Hit
TGCAGGTTCAAAGGGAGCAGGCTGAAAGACATGTAAGTACTTTTTATCTT20.18674136321195145No Hit
AGATTTGCAAAACAACCTTGCCCCTCTTCCAAGCTTGCATTAAACAAGAT20.18674136321195145No Hit
ACAAATTGAAGCCCTGGAAAGAGACAAAGTAGAAAGATCGGAAGAGCGTC20.18674136321195145No Hit
TGCAGGCCCAATACTTTAGGGGGTGAAGAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
GGGTTTCAGGTGCGCGTGGTTCGGGTGCGGCGCGCCGGGAGATCGGGAAG20.18674136321195145No Hit
GTGAGTGAGCCTACGACCACCAATTGCTTATCTCCTATTACCAATNGCAG20.18674136321195145No Hit
TCAATTTATGACCTTTAAGTCATTTCGACTTTTGTCAAAGTCAAAATAGT20.18674136321195145No Hit
GAGAGGGCGGAGATGGCGGTGGAGAGGGCGGAGATCGGAAGAGCGTCGTG20.18674136321195145No Hit
TTGGGAATTGGGGCCTTCTTTGCCTTTGGTGCCATAGATCGGAAGAGCGT20.18674136321195145No Hit
AATTAGAGAAAATCCTGCAAATTCTAAGAAAAAACAAACTAAATAAGATC20.18674136321195145No Hit
CCTGATTAACTACCAGCTAGCTGGCTTATTTGAGATCGGAAGAGCGTCGT20.18674136321195145No Hit
CTCTCCCACGTTCGATCGGAGTACTATGCTGTGGGCAGATCGGAAGAGCG20.18674136321195145No Hit
CTGTGCCCGAGCTAGACGCCGGCTGGTGGCCACCCAGCCGCCTAGCCTTA20.18674136321195145No Hit
GTTCCCGAATTTACCAAGTTTAATGGTGAGGATAGTAGGACCACATAGGA20.18674136321195145No Hit
TGGCCTCCATTGCCATCCTGTCTTCTTGCTCATCAAGATCGGAAGAGCGT20.18674136321195145No Hit
ATTATTGCGTCAGCCGAGGGGCGGGCGAGATCGGAAGAGCGTCGTGTAGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 23bp)
CGGAATTAACTGGCTGCACGTTAAATCGAAAAAATGAGATCGGAAGAGCG20.18674136321195145No Hit
TTTGACTTGCTCTCTAGAGAGTTTGGGACGACGAGAAAATGACACAGATC20.18674136321195145No Hit
CGCCTTTCGTGCGGGTCGGAACTTACCAGATCGGAAGAGCGTCGTGTAGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 23bp)
TGTCCGGTGAGATTTGGGGGGAACGAGATCGGAAGAGCGTCGTGTAGGGA20.18674136321195145Illumina Single End PCR Primer 1 (100% over 25bp)
CTTTCTCAAAAGTAAGACCATAATCTTTCAAAGTAGATAAAAGCCAAAGA20.18674136321195145No Hit
AGGAGCCAGACAAAAAGATCACAGTGAAGAAGAAAATCTAGCAGAGATCG20.18674136321195145No Hit
GTTCCGAATATGAGAAGGGGCTCGCGGATTTTAACAGGTTAGGCTTAATC20.18674136321195145No Hit
TCCTGGTATGTAGGGCTCACGTGAGTCCCACGCTGACTCAGCCACCATGT20.18674136321195145No Hit
CACACACTAGAACGCCAGATCATCAACATCTGCAGAAAATAGATCGGAAG20.18674136321195145No Hit
TATTTCTAATATATTTTTTGAGCAATTCTACGGTCCTTGAGGAGGTACCA20.18674136321195145No Hit
TTTTGTTCTTTTCCGCTTCAATAGCTGGTTCGGTCGATCCGAATAGATCG20.18674136321195145No Hit
ACTTTATTATATATATATGTTTTCAGAATATGGCTCCGGATCTCAGGTCT20.18674136321195145No Hit
TCACCTCACTCGTTTTCCACGTGCATGCTTTTCAAACTAAGATCGGAAGA20.18674136321195145No Hit
GGAACGTTTGTATAGCGCCGGGAGGGAGAGAGGGCGGCCGGGGAAGGAGG20.18674136321195145No Hit
TTCGTGCTCAAGTCCCAGTCCTCAAGTGGTTCTGGTCGGGGCTGGTGCCT20.18674136321195145No Hit
TGTTATCAAATTTTGGCAGGTTTTTGAGATCGGAAGAGCGTCGTGTAGGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 24bp)
TGCCCAACCTTCCGGTCTCTTAGCGTGGCGCGCAATGACAAGGCCGTAGC20.18674136321195145No Hit
ATTTCCGCCGCACGATCCACCGCAGATCGGAAGAGCGTCGTGTAGGGAAA20.18674136321195145Illumina Single End PCR Primer 1 (100% over 27bp)
TTTGAACCGAAGTCGAACAAAGGGAAATATCAGCAGATCGGAAGAGCGTC20.18674136321195145No Hit
GCCCACCTAGCATATATTCCTTGTCCTAGAAGCTCCAGATCGGAAGAGCG20.18674136321195145No Hit
TCTTACCATAGAAATACACAAAGGCAGGGTGCTTCTGGGGGTGACAGATC20.18674136321195145No Hit
CCGGGCGAGAGGGCGCGCGGACCGGGTCCACGGGAAAGATCGGAAGAGCG20.18674136321195145No Hit
AGAAAAAGGAATTTCCCTGGAATTTAAAATTGCACTAGATCGGAAGAGCG20.18674136321195145No Hit
AGACGAGAGCAGCGAGCTAGGAGGAGGCCATGGCCGGCGGCGAGCAGATC20.18674136321195145No Hit
CCTGGAGGCATTTTGTTAAGATTCTTATTTTGAGTTACACTGAAAAGATC20.18674136321195145No Hit
CTCTTTGACCACATTGCAATGCACCTGCCTGCCGTAAGAGATCGGAAGAG20.18674136321195145No Hit
GTGATTTACAAGAATCTAATGAGAACACAATGACTAGATTTTTCAGAGGG20.18674136321195145No Hit
AAAACTATTTTGATCACTTCCATTTTAGATCGGAAGAGCGTCGTGTAGGG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 24bp)
CCTCCCTAAGTGCGCGTTGCTCGGGAACGAGATCGGAAGAGCGTCGTGTA20.18674136321195145Illumina Single End PCR Primer 1 (100% over 21bp)
ATACGCAAGAGTCGAATCAACCAGCAGAACAAGGGGAAAACTTGGAGAAG20.18674136321195145No Hit
CTCAGGCTTGTAACGCGGCATCTAAGACAAGTGAAAAGAGGGAGTTAAGA20.18674136321195145No Hit
GTTTATTTTTCAGCCTTTGCTTTTAGATCATTAAGAGCACGAGATCGGAA20.18674136321195145No Hit
TACTTGTTCCACGAAAGCAAAACACGTAAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
CAAGAGTGTATAGCCAACTTGTAAGGCATTGCAGATCGGAAGAGCGTCGT20.18674136321195145No Hit
GTCTTAGATTTTGCATTTTGATGAGCCTCGTTTTGAGGAATTCATGGAAT20.18674136321195145No Hit
TCCAGTCATCAGCGGTGGGTGGATTTGGAGATCGGAAGAGCGTCGTGTAG20.18674136321195145Illumina Single End PCR Primer 1 (100% over 22bp)
CGGCCTTCTCGCCGGCCGGCTTCTGCTCCCCTTTCAGCTAGATCGGAAGA20.18674136321195145No Hit
TGCCGCCGCTGATTGCGCCTTTCGGTGATATGCGCCTTTCGGTGATAGGT20.18674136321195145No Hit
CTCATTGCTCCGGAAGAGCAAGTTTAGTCTCATCAGATCGGAAGAGCGTC20.18674136321195145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)