FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SNW-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SNW-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8181240
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA7590629.278080095437856Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA7354028.988881880986256Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA7082008.656389495968826Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA4189405.120739643379244Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3580994.377074868846288TruSeq Adapter, Index 12 (100% over 50bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA1934302.364311522458698Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA1129851.3810253702372746Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG991151.2114911675980657Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG981211.1993414201270223Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG654910.8005021243723445No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA354950.4338584371073334No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG348070.42544895394830123Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG334440.40878888774806754Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA332480.4063931628946223No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG328220.40118612826417516Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA302850.37017615911524415No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC292690.3577575037524874No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA253240.3095374295339093No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC218440.26700109029927005No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC202390.24738303729019076No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT191630.23423099676821607TruSeq Adapter, Index 1 (97% over 35bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC183080.22378025824936074No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA177300.21671531454889478No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG172890.21132493362864307Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG168670.20616679134214375Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA162460.1985762549442383Clontech Universal Primer Mix Long (95% over 24bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA158470.1936992436354391Clontech Universal Primer Mix Long (95% over 22bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG158360.19356478968958252No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA153650.18780771618972184Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC148920.18202619651788726No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA141670.17316445917733742Clontech Universal Primer Mix Long (95% over 22bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG137980.16865414044814722No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG122020.1491460952129506No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC109440.13376945304134827No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA108310.13238824432482119No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA106840.13059145068473726Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC104710.12798793336951367No Hit
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA103350.1263255936752864Clontech Universal Primer Mix Long (95% over 24bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA101550.12412543819763262Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCACA98120.11993291970410352No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC92260.11277019131574187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG92230.11273352205778096Clontech Universal Primer Mix Long (95% over 22bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC91430.11175567517882376No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA89970.10997110462472681No Hit
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT87910.10745314891141196TruSeq Adapter, Index 1 (97% over 35bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA87860.10739203348147713Clontech Universal Primer Mix Long (95% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC87540.10700089472989426No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC87360.10678087918212886Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACACG82710.10109714419819002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)