FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SNW-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SNW-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19851708
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA247373312.46105876632882Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA241222012.151196259787824Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA237267611.951999293965034Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA14508497.308434115593479Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC7557703.8070779602440257TruSeq Adapter, Index 10 (100% over 50bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA6555183.302073554577772Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA3590361.8085899712004627Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG3458301.7420667279611408Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG3399541.7124672597440986Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG2258951.1379121635276923No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA1280230.6448966507063271No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA1192750.6008299134764625No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG1120900.5646365541947322Clontech Universal Primer Mix Long (95% over 21bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC1078690.5433739001198284No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA1072140.5400744359125169No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG1064020.5359841077654376Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG943340.47519336875194823Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA833350.4197875568187886No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC807960.40699772533426337No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC799760.4028670983877055No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA738330.37192265773806465No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG714490.35991361549343764No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC686120.3456226537283341No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG604160.30433653366249397Clontech Universal Primer Mix Long (95% over 22bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA527320.26562953676328505Clontech Universal Primer Mix Long (95% over 22bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG524170.2640427715338146No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC514670.2592572890957292No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG510350.25708115392388403Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA508260.2560283477875053TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC482810.2432082922033711TruSeq Adapter, Index 10 (98% over 50bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA464820.23414609967061778Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG460520.23198003919864224No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA444380.22384975640383187No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC424140.21365416013574248No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC413540.2083145692048261No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC411700.20738769681681798TruSeq Adapter, Index 10 (98% over 50bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC406170.20460204230285878No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA388310.19560533531925817No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCACA376040.18942450694922575No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC374900.18885024905665548No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA368650.18570190534738876Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA366140.18443753051374723Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACACG356280.17947070347800803No Hit
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA352660.17764718280160074Clontech Universal Primer Mix Long (95% over 24bp)
GGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCC350790.17670519836378815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCAC340350.171446205031829No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACAC337720.17012138199896956No Hit
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA328070.16526033931186174Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA319090.1607367990703873Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG307940.15512015389305545Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA306530.15440988755224488Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA299940.15109027394519403Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC289550.14585646736290903Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA278380.14022974748570755Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGAGATCGGAAGAGCAC274070.1380586496637972No Hit
CCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA269120.13556516144605796Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGAGATCGGAAGAGCA265840.13391291066743477No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG262560.13226065988881158Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCCGGCCGAGGAGATCGGAAG261470.13171158874591546Clontech Universal Primer Mix Long (95% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCA248900.12537963987783823No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT245610.1237223517492802Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC244340.12308260830755723Clontech Universal Primer Mix Long (95% over 24bp)
GCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCA232470.11710327393491785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA210240.10590524502979795TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)