FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SNN-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SNN-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13497244
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA144563410.710586546409031Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA141969810.518428799242274Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA13154939.746382298489973Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA7682385.691813825103851Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4705183.4860301851252005TruSeq Adapter, Index 9 (100% over 50bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA4121443.0535418934413574Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA2414201.788661448218614Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG2013151.4915267146389293Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG1982071.4684997915129934Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG1366951.0127623091054738No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG1044490.7738542772139261Clontech Universal Primer Mix Long (100% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG816510.6049457207708477Clontech Universal Primer Mix Long (95% over 22bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA797290.590705776675594No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG711580.5272039240010775Clontech Universal Primer Mix Long (95% over 21bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC669590.49609386923730503No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA654910.4852175747878604No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA595090.44089741579836594No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA538260.3987925238663538No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC447630.33164548258889004No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA436000.32302890871647577TruSeq Adapter, Index 7 (97% over 35bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG400630.2968235589428479Clontech Universal Primer Mix Long (95% over 23bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC383860.2843988002291431No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA369220.2735521414593972Clontech Universal Primer Mix Long (95% over 22bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC362220.2683658975121143No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG351500.26042353535284685Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC328720.24354601576440346No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA321930.2385153591355391Clontech Universal Primer Mix Long (95% over 22bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA319670.23684094323255916No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG299440.2218526982249117No Hit
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA277080.20528635327330527Clontech Universal Primer Mix Long (95% over 24bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG259940.19258746452238695No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA257450.19074264346113917Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG257140.19051296694347378No Hit
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA243050.18007379876958585Clontech Universal Primer Mix Long (95% over 24bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC200280.14838584825168752No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA192820.14285879398786894Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA192700.14276988694877263Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG183280.13579068437971487Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG183070.13563509706129637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC181320.13433853607447566No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA179280.1328271164098389No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC178720.1324122168940563Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAA178600.13232330985496002Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA170590.12638876499528348Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA167230.1238993679005877TruSeq Adapter, Index 7 (97% over 35bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA166200.12313624914834466Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCACA164470.12185450600137332No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC161870.11992818682095396TruSeq Adapter, Index 9 (100% over 50bp)
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA157380.11660158177476823Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA153070.11340833728722692Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC151040.11190432654251489Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA151020.11188950870266552No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG150870.11177837490379516Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC149190.11053367635644729No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG146710.1086962642151242Clontech Universal Primer Mix Long (100% over 23bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG144480.10704407507191839TruSeq Adapter, Index 9 (97% over 49bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG144130.10678476287455424Clontech Universal Primer Mix Long (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)