FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SNN-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SNN-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114939
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1349424.33209125443548TruSeq Adapter, Index 8 (100% over 50bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA509861.636821780458622Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA469021.505711668832038Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA394641.266926896481761Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA274030.8797283028656421Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC130480.41888460737112343TruSeq Adapter, Index 8 (98% over 50bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA94390.3030235905101192Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC93590.30045532191802155TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG70150.22520505216956094TruSeq Adapter, Index 8 (97% over 49bp)
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA66900.2147714610141643No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG53230.17088617144669607Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA48120.15448135581467246Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG44340.14234628671701113Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG43170.13859019390106836No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC40870.13120642169878768TruSeq Adapter, Index 8 (98% over 50bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG38220.12269903198746429Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCCTCGAACCAATTGCGGGGTTCTATCCCCGATTT35920.11531525978518359No Hit
GGTCGCGGCCGAGGATCCAGATTCAGCATGGAGAATTATGACCACACTCA32490.104303808196565No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)