FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SNN-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SNN-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9562114
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA6731587.039844954787195Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA6066556.344360671709206Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA5732585.995096900120622Clontech Universal Primer Mix Long (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3749463.921162203253381TruSeq Adapter, Index 7 (100% over 50bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA3033283.1721855648238457Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA2020342.1128591439089726Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA1337491.398738814450445Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG1043581.0913695444333753Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG900170.9413922486178266Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG501050.5239950077984847No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG434390.454282389856469Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG378170.39548785969295075Clontech Universal Primer Mix Long (100% over 23bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA368700.38558419194751287No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC297620.31124916519506046No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG278400.29114900742660044Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA275530.2881475790813621No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG233090.24376408815038178Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA217530.22749153586748705No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC216110.22600650860259563No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA215360.2252221632162093No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA181790.1901148637215578Clontech Universal Primer Mix Long (95% over 22bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC181230.18952921916638935No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA175430.18346361484500184Clontech Universal Primer Mix Long (95% over 22bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA173410.18135111127100137No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC168510.17622672141327744No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG166150.17375864793078183No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC152480.15946264602158058No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG143810.15039561335495477Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC142330.14884783845915242TruSeq Adapter, Index 7 (98% over 50bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA139890.1462961014687756TruSeq Adapter, Index 7 (100% over 35bp)
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA134320.14047102973254658Clontech Universal Primer Mix Long (95% over 24bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG121110.1266560929936623No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC112550.11770409765037312No Hit
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA112290.1174321912497592Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG110320.11537197736818448No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC99000.1035335910029937Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA98970.10350221718753824Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC97660.10213222724598348Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA96910.10134788185959716Clontech Universal Primer Mix Long (95% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC96350.1007622373044287TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)