FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SEW-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SEW-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14094190
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA152506010.820487023376298Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA150414010.672057067486673Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA13115979.305940958650337Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA8071835.727062002144146Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA4222392.9958372918202465Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3587642.545474411796634TruSeq Adapter, Index 6 (100% over 50bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA2440341.7314510447212648Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG1942611.378305528732052Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG1902141.3495915692920275Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG1278770.9073029383029461No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG813230.5769966205933083Clontech Universal Primer Mix Long (100% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG805680.5716398033515938Clontech Universal Primer Mix Long (95% over 22bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA698440.4955517131527246No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG663530.47078264164169775Clontech Universal Primer Mix Long (95% over 21bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC593680.4212232132531206No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA579360.4110629982992992No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA514270.36488084806576326No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA483170.34281501810320425No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC456640.3239916589743717Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG397550.28206658204550955Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC382380.27130328170685936No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC376380.26704620840218557Clontech Universal Primer Mix Long (95% over 24bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA348960.24759138339982648Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG348210.24705924923674222Clontech Universal Primer Mix Long (100% over 23bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG347350.24644906872973898Clontech Universal Primer Mix Long (95% over 22bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC321740.22827846084095643No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA294110.20867463827293373Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC291610.20690085772931965No Hit
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA258200.18319605454446122Clontech Universal Primer Mix Long (95% over 24bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA255520.18129456180170694No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC248950.17663306653308916TruSeq Adapter, Index 5 (97% over 35bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG245640.17428458109334413No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC244490.17346864204328166No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA225010.15964734404744083Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG209080.14834481442353198No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG204700.1452371509111201No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA187100.13274973588407707Clontech Universal Primer Mix Long (95% over 23bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA184530.1309262894852418Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG184510.13091209924089287Clontech Universal Primer Mix Long (95% over 22bp)
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA178800.12686078447927834Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA167500.11884329642214274Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA158250.11228030841077068Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA153460.10888174488920613Clontech Universal Primer Mix Long (95% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC153010.10856246439135558No Hit
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA145700.10337593008182805Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC141940.10070816414423248Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)