FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SEN-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SEN-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9783877
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA115865611.842503743659083Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA109747511.21717903853452Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA104838110.715394316588403Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC7499387.665039125083033TruSeq Adapter, Index 2 (100% over 50bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA5985226.11743177065697Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA2898442.9624656973917394Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA1796111.8357855479990193Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG1585461.620482350708211Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG1488981.5218711355426893Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG865760.884884386833563No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA523920.5354932405630202No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG497500.5084896304399575Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG486550.49729774812173133Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA446320.45617907911148103No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG435150.4447623370571809Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA428950.43842538085873317No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC418710.4279591822342002No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA377180.3855117966016948No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC331750.3390782611024239No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC327710.33494901867633864No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA311560.3184422698690918No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG274370.2804307535754998No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC273440.27948021014573265No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG245640.2510661162236606Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG241650.2469879782830467Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG217070.2218650132253298TruSeq Adapter, Index 2 (100% over 35bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC210120.2147614897448118No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA208040.21263554314920352Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA206560.2111228503792515Clontech Universal Primer Mix Long (95% over 22bp)
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA202570.2070447124386376Clontech Universal Primer Mix Long (95% over 24bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG199850.204264628428996No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC192710.19696690790368684TruSeq Adapter, Index 2 (98% over 50bp)
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA188690.19285810727179012Clontech Universal Primer Mix Long (95% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC188160.19231639972579379No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA176030.17991845155044367No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG175060.17892702453229942No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC169050.17278426537864286Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC168400.17211990706751526No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG166610.17029036648764084Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCACA165460.16911496332179973No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC158880.16238961303376973Clontech Universal Primer Mix Long (95% over 24bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC153520.15691121219124077No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC153350.15673745694063815TruSeq Adapter, Index 2 (98% over 50bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC150750.15408002369612783No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA149640.15294550411866378No Hit
GGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCC148740.15202562338017944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA145320.14853007657393894Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCCGGCCGAGGAGATCGGAAG145030.1482336705582051Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG139710.14279615330405318Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA139630.14271438612729903Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA138660.14172295910915478Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACACG138170.14122213515153553No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCAC136240.13924950201234132No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA135660.13865668998087363Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA132200.13512025958625604Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA129450.13230951288533166Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGAGATCGGAAGAGCAC125820.12859932724011147No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA125730.12850733916626303Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACAC120840.12350932048716474No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGAGATCGGAAGAGCA115840.11839887194002949No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCA106240.10858681072952982No Hit
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAAGATCGGA100100.10231117991364772Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC99920.10212720376595087Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)