FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_SEN-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_SEN-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673128
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA129347712.119005787244378Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA126346111.837776142101921Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA120258711.26742788056135Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA7500577.027527450247013Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC6105885.7207971271402345TruSeq Adapter, Index 1 (100% over 50bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA3118482.9218051165506496Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA1905761.7855683919465781Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG1705031.5974979406224679Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG1700371.5931318353907122Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG1116311.0459070667942894Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG982300.9203487487454474No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG558540.5233142523916138Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA532740.49914139509991823No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG512430.4801122969761067Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA501660.4700215344555036No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA498150.46673290154488917No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC435260.4078092195652483No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC388560.36405447400237306Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA361130.3383544168120161No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC326610.30601150852870873No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG317840.2977946109144386Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC309460.28994311695690334Clontech Universal Primer Mix Long (95% over 24bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC278160.26061713117279206No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG272270.2550985990236414Clontech Universal Primer Mix Long (95% over 23bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG268530.2515944716487987Clontech Universal Primer Mix Long (95% over 22bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA265750.2489897994289959Clontech Universal Primer Mix Long (95% over 22bp)
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA239430.22432973726165376Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA233140.2184364321312365Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC229540.21506347529983713No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG219880.20601270780224878No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA201120.18843585498084536No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA197440.18498794355319265Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA196190.18381677798673454Clontech Universal Primer Mix Long (95% over 24bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC192970.18079985548753844No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG192010.17990040033249857No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAA186100.17436312953428462Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT184300.1726766511185849TruSeq Adapter, Index 1 (100% over 35bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA171650.16082445558602876Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGATCGGA165500.15506232099905481Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG161410.15123026726560385No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAAG150110.14064293054482246Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA147610.13830059941190623Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA132320.12397490220299053Clontech Universal Primer Mix Long (95% over 24bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC130000.12180121891164426No Hit
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA129420.1212577980888077Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCTTGGTCGCGGCCGAGGAGATCGGAA124430.11658250514750688Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGGGTCGCG118480.11100775705116625Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC117550.11013640986972141Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGGGTCGCGG116690.10933064795999824Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCACA112020.1049551734037107No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA110990.1039901329769492No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC107650.10086077858337311No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC107130.10037357370772655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)