FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNW-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNW-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10057559
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA4846184.818445509491916Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA4687214.660385288318965Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA4568664.54251374513438Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2737002.7213362606175115Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1076751.0705878036609082TruSeq Adapter, Index 25 (97% over 44bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA953210.9477548180428274Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA502240.49936570096183375Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG491150.4883391685795728Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG474890.47217222389647434Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG343400.3414347358041847No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG265130.2636126718222582Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG246090.2446816369657886No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT229530.22821640917045577No Hit
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC219350.21809466889530552No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA209280.20808229909464118No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG182620.18157487318742052No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA175480.17447573511624442No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA175020.17401836767748516No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG173200.1722087834632638Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA165550.1646025641012894No Hit
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG165260.16431422375946292No Hit
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA163510.1625742389380962No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC156450.15555464303018257No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG149960.14910178503551408No Hit
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC137150.13636509614310988No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG133180.13241781629120944Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCGGTTGATTCTTTGAAGTATCGAAGCGCCTGAGT130440.12969349719946957No Hit
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA127320.12659135283223294No Hit
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA120770.12007883821511761No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC120560.1198700400365536TruSeq Adapter, Index 5 (100% over 35bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA110080.10945001664916905No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG108820.10819722757778502Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT106990.10637770059315585No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC105640.10503542658810154No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC102960.10237076411880855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)