FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNW-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNW-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733309
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA5338284.549679889961135Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA4784224.077468683386758Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA4571193.8959086477650935Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2898002.469891485854502Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT1063660.9065302891111109TruSeq Adapter, Index 7 (97% over 36bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA943490.8041124630741421Clontech Universal Primer Mix Long (95% over 21bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG519740.4429611459137401Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG500160.4262736113060689Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG466700.39775650671093726No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA466540.3976201427917734Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC435670.37131042913810586No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG366460.3123245113548105No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT363280.30961427846142975No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG322070.27449204653180104No Hit
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA319010.27188408657779317No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG318140.27114260776733995No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA286870.24449198431576294No Hit
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG275430.23474196409555056No Hit
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA274120.23362548450739684No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC202510.17259410793664431No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA200930.1712475142349017No Hit
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC195490.16661114098333213No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA190530.16238385948925405No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG186710.1591281709192181Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT181890.15502020785440834No Hit
GGTCGCGGCCGAGGATCGACTTCGCTTGTGACCATTGCTTGGTGGTAACT180590.15391225101120237No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG177970.15167929183489498Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA177510.15128724556729903TruSeq Adapter, Index 7 (97% over 35bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC173580.14793780680283797No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA172640.14713666877775058No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA169080.14410257157635584No Hit
GGTCGCGGCCGAGGATCAAAAGATTTAAGAAATCTCTGCCCCACTGCAGC163030.1389463108829743No Hit
GGTCGCGGCCGAGGATCCATGTCAGCATGGGAGCGTTCTTGCCAATGGTG156530.13340652666694452No Hit
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG147930.12607696601188975No Hit
GGTCGCGGCCGAGGATCAAGACAGGCATCTCTAAAGCCCCAGACATGAGC146440.12480707701467675No Hit
GGTCGCGGCCGAGGATCCTGCATCAACTTTCAGCCTCTTAGCCACTTCAT137040.11679569676380296No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG133000.11335250780491676Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA132530.11295193879237307No Hit
GGTCGCGGCCGAGGATCAGAAAAACGCTACCTGCTCCTACGAAAGGTTCC118400.10090930018121912No Hit
GGTCGCGGCCGAGGATCGCATGCAACATTTGCAATGGCCTCTTCCTTTGG118050.1006110041080483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)