FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNW-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNW-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8805297
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA4433065.034537733366632Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA4006354.54993170588113Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3943934.479042558132906Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2364032.684781671759624Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA868710.9865766026972175Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT778150.8837294187805362TruSeq Adapter, Index 22 (97% over 40bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG499020.5667270507740966Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA486680.5527127591494074Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG466580.5298855904576529Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG274760.3120394462560434No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG253900.288349160738133No Hit
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC226430.2571520301927351No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT201870.2292597285474868No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG198320.2252280644253113No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG177800.20192391011910216No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA170560.1937015866699329No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG158780.18032327586451655Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA158570.18008478305728928No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA148240.16835320830177564No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG146030.16584335542571704Clontech Universal Primer Mix Long (95% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA145150.16484395699543128No Hit
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA140720.15981289444296995No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA137860.1565648495445412No Hit
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG134080.15227197901445005No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC131090.14887629571154726No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG123350.14008613224517016Clontech Universal Primer Mix Long (95% over 22bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC122160.13873467300421552No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG117450.13338562004211785TruSeq Adapter, Index 2 (100% over 35bp)
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC117000.13287456402663078No Hit
GGTCGCGGCCGAGGATCAAAAGATTTAAGAAATCTCTGCCCCACTGCAGC104760.11897384040538325No Hit
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT102920.11688418914205846No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA99110.11255724821093485No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC93690.10640186242440203No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG91440.10384658234696682Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA90640.10293803831943431No Hit
GGTCGCGGCCGAGGATCGACTTCGCTTGTGACCATTGCTTGGTGGTAACT89990.10219984629706415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)