FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNN-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNN-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8811128
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA4054154.601170247441645Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA3859804.380596899738603Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3654654.1477663245840946Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2023982.2970725201132023Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA850230.9649502311168332Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA532480.6043267104960909Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG469670.5330418534380615Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG426390.48392214935477046Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG277470.3149086019406369Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG259410.2944117938134595No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT250780.28461736113696223TruSeq Adapter, Index 21 (97% over 40bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG247260.2806224129305578No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG219260.24884441583415878No Hit
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC197850.22454559734009083No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT181620.2061257083088567No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG161870.1837108710712181No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA157320.17854694654305328No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG150380.17067054297701723Clontech Universal Primer Mix Long (95% over 21bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA145710.16537042703272498No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG134120.15221660609175125Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT130180.1477449879289008No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA129160.1465873608918177No Hit
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG127660.1448849681902249No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA126570.1436478961604008No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC123860.14057224001285648No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA121280.13764412456611685No Hit
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA120950.13726959817176643No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC119780.13594173186452405No Hit
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA119770.13593038257984677No Hit
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC109850.12467189217997968No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA103180.11710191930023035No Hit
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA101010.11463912452525941No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC91750.10412968691409316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)