FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNN-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNN-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8126272
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA3582724.408811322092098Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3064283.7708312003339293Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA2953093.634003390484591Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2041722.512492813432777Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1157051.4238386310475455TruSeq Adapter, Index 20 (97% over 44bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA578260.7115932127302654Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG333820.4107910736928323Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG329210.4051181156623849Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC306990.3777747040709442No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA303150.37304929000653686Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG274420.33769482488403046No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG223840.2754522615044143No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG222080.27328644672489427No Hit
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA210880.25950398903703936No Hit
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA204660.25184980271396284No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT190400.23430178069353325No Hit
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG187190.2303516298740677No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG171670.2112530813637545Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG167590.20623232892032164No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA162110.19948876926590692No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT133450.16422044450394965TruSeq Adapter, Index 1 (97% over 35bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA128670.15833828845502587No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA127960.15746457908374223No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA126160.15524954124105125No Hit
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT125490.1544250549329385No Hit
GGTCGCGGCCGAGGATCAAAAGATTTAAGAAATCTCTGCCCCACTGCAGC117550.1446542768935128No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG116880.14382979058540005Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC112040.1378737999417199No Hit
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC110680.13620021579390895No Hit
GGTCGCGGCCGAGGATCCATGTCAGCATGGGAGCGTTCTTGCCAATGGTG105400.12970277145534878No Hit
GGTCGCGGCCGAGGATCGACTTCGCTTGTGACCATTGCTTGGTGGTAACT105040.1292597638868106No Hit
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG101460.12485429973301411No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC99480.12241775810605406No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA97520.12000582801067942No Hit
GGTCGCGGCCGAGGATCAAGACAGGCATCTCTAAAGCCCCAGACATGAGC95270.11723703070731573No Hit
GGTCGCGGCCGAGGATCGCATGCAACATTTGCAATGGCCTCTTCCTTTGG82210.10116570058201349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)