FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NNN-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NNN-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8859342
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA5174935.841212586668401Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3993114.507230898186344Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA3925064.430419324595438Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2763163.1189223759507194Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1198101.3523577710398809TruSeq Adapter, Index 19 (97% over 40bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA786870.8881810861348394Clontech Universal Primer Mix Long (95% over 21bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG485690.5482235588150903Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG482600.5447357151355032Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA399970.45146693738654636Clontech Universal Primer Mix Long (95% over 24bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG317920.3588528357975118No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG280380.3164794857225288No Hit
GGTCGCGGCCGAGGATCCAAACCACCTGAGCTACCAAACACCAATAATTC249350.2814543111666758No Hit
GGTCGCGGCCGAGGATCCACAACACCTATGCAGGACTGACCAGAGGGATT206810.23343720109236105No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG203650.22987034477278337No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG199780.22550207453330057No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA196130.22138212973378835No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA189840.21428227965462898No Hit
GGTCGCGGCCGAGGATCCATCCCTTGGACACACGGTCAAACATCAGCACA183240.206832516455511No Hit
GGTCGCGGCCGAGGATCTTAATCCTAATGGTAATGGGCTTAATCAATACA171320.19337779261710408No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT169290.19108642605737539TruSeq Adapter, Index 1 (97% over 35bp)
GGTCGCGGCCGAGGATCGGCATTGCGAAGGAAGCTGCAGATACATCATGG165630.1869551937378645No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG159260.1797650434987158Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCATCTAATTGGTTACCGTCACATGAGCCATGAGA152140.17172832925966736No Hit
GGTCGCGGCCGAGGATCGGTTGATTCTTTGAAGTATCGAAGCGCCTGAGT151500.1710059279797529No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC143590.16207749966081003No Hit
GGTCGCGGCCGAGGATCATGGCCACTAGACCAAATCCCTTACTTATCCTC141740.15998930846105727No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA140540.15863480606121763No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC139970.1579914174212938No Hit
GGTCGCGGCCGAGGATCGACTTCGCTTGTGACCATTGCTTGGTGGTAACT123810.1397507851034535No Hit
GGTCGCGGCCGAGGATCCTTCTTTCCGTCTCGCCGAGTATCCTCTCTTCC115090.1299080676646189No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC109790.12392568206532718No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC107090.12087805166568805No Hit
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA102690.11591154286627607No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG101950.11507626638637496Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG101550.11462476558642842Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT100380.11330412574658479No Hit
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG99570.11238983662669305No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA97790.11038065806693093No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA93630.10568504974748688No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG91430.10320179534778091Clontech Universal Primer Mix Long (95% over 22bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG90880.10258098174785442No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA90620.10228750622788915Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA90000.10158767998797201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)