FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NEW-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NEW-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10312514
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA5093224.938873295105345Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3759383.645454444958814Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA3574453.466128627801136Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2940472.8513609775463093Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1608691.5599397004454976TruSeq Adapter, Index 27 (97% over 44bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA668290.6480379081182338Clontech Universal Primer Mix Long (95% over 21bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG481150.466569063566847Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG452750.4390297070142159Clontech Universal Primer Mix Long (100% over 23bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG366750.3556358808337133No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA340960.3306274299360951Clontech Universal Primer Mix Long (95% over 24bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG276930.2685378172577511No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG234870.22775241808156577No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG219560.21290637763012976No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA193870.18799489629783775No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA184120.1785403636785366No Hit
GGTCGCGGCCGAGGATCAGAAAGAATGTATCCTGAAGACGGAGCTCTCAA173740.1684749227976806No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT146270.14183738320258282TruSeq Adapter, Index 1 (100% over 35bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG139530.13530163449959923Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT133530.12948346058002927No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC130210.12626407101120057No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA126310.12248225796348011No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC124080.12031983665670659No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA117850.11427863273688647No Hit
GGTCGCGGCCGAGGATCCAGATTCAGCATGGAGAATTATGACCACACTCA107640.10437804011708493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)