FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NEW-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NEW-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5798940
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA2335394.027270501160557Clontech Universal Primer Mix Long (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT1715472.9582475417921206TruSeq Adapter, Index 16 (97% over 39bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA1613702.782749950853087Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA1550502.6737645155838825Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA1244332.145788713109637Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA304750.5255270790868677Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC241630.4166796000648394TruSeq Adapter, Index 5 (97% over 35bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG211250.36429071519967443No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG208820.3601002941916971Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG197410.34042428443819045Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA166070.2863799246069109Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG156990.2707218905524113No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG145120.25025263237764145No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG129210.22281658372047308No Hit
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT107810.1859132876008374No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA107110.18470617043804557No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCGCGTAT104970.181015840826082TruSeq Adapter, Index 16 (97% over 39bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA100320.17299713395896493No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA93310.16090871780014968No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTA90920.15678727491576047TruSeq Adapter, Index 16 (97% over 39bp)
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC89730.15473517573901435No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC78980.1361973050247114Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG78510.13538681207255118Clontech Universal Primer Mix Long (95% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG76690.13224830744929245TruSeq Adapter, Index 16 (97% over 38bp)
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG76640.13216208479480732No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC71170.1227293263941341No Hit
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC70730.12197056703466494TruSeq Adapter, Index 5 (97% over 35bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC64680.11153762584196422Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA63410.10934757041804193No Hit
GGTCGCGGCCGAGGATCAGAAAAACGCTACCTGCTCCTACGAAAGGTTCC60230.10386380959278765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)