FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NEN-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NEN-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7570489
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA6230008.22932309920799Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA4658276.153195652222729Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA4525315.977566310445733Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA3335504.405924108733267Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1355341.7902938634479226TruSeq Adapter, Index 15 (97% over 40bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA929891.2283090299715118Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG616570.8144388030944897Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG612360.8088777356390057Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA481840.6364714353326449Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT441410.583066694899101TruSeq Adapter, Index 1 (100% over 35bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG384610.508038516402309No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG295520.39035787516499926No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA251610.3323563378798913No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA248830.32868418407318206No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG223420.29511964154495174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT205360.27126385098769706TruSeq Adapter, Index 15 (97% over 40bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG196990.2602077620085044Clontech Universal Primer Mix Long (95% over 21bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC195490.25822638405524395No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA182870.2415563908751469No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC176930.23371013418023592No Hit
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT173910.22972095990100508TruSeq Adapter, Index 1 (100% over 35bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA169030.2232748769597314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT153520.20278742892301937TruSeq Adapter, Index 15 (97% over 40bp)
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA152060.2008588877151793Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG151850.20058149480172283No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA149600.1976094278718323No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC149490.19746412682192654No Hit
GGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTC149120.196975386926789Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG148140.19568088666399225No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT136720.18059599584650343TruSeq Adapter, Index 15 (97% over 40bp)
GGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCC130040.17177225936131735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG127920.16897191185404273Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA126740.16741322786414456No Hit
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC120800.15956697116923357No Hit
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT119420.15774410345223408No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA118800.15692513389821977No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG116820.15430971499991614No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA116410.1537681383593583No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA114920.151799969592453No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG114540.1512980205109604No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG113450.14985821919825787Clontech Universal Primer Mix Long (95% over 22bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTG110360.14577658061454155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC105500.13935691604597802No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC104270.13773218612430452No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACAC98310.1298595110566834No Hit
TCCTCGGCCGCGACCGCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA97610.12893486801182855Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCAC96130.12697990843127835No Hit
GGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACT94620.12498532129166293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC94230.12447016302381525No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC93690.1237568669606415No Hit
AGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACT92700.12244915751148967Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC91260.12054703467635974No Hit
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG89660.11843356485954872No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA87060.11499917640723076Clontech Universal Primer Mix Long (95% over 22bp)
TAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC85510.11295175252219505Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG84250.11128739504145638Clontech Universal Primer Mix Long (100% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCAC82890.109490945697167No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAA82680.10921355278371053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTC82540.10902862417473957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTGAGATCGGAA82260.10865876695679763Clontech Universal Primer Mix Long (95% over 21bp)
ATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC81640.10783979740278336Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA78450.10362606695551635Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAA78220.10332225566934976Clontech Universal Primer Mix Long (95% over 22bp)
TCCTCGGCCGCGACCAAGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA76540.10110311236169815Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACACG75910.10027093362132881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)