FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NEN-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NEN-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132287
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT3126576.091962511059884TruSeq Adapter, Index 14 (97% over 44bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA3123886.086721182973594Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA2423484.722027431435538Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA2158994.2066821282597795Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA1469972.864161727510562Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG581871.1337440793938451TruSeq Adapter, Index 2 (97% over 35bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA472040.9197459144432102Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG328780.6406110959889811Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG287280.5597504582265178Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA281420.5483325464846374Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA203830.39715238060537145Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG195450.380824377124662No Hit
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG193600.37721974628464855TruSeq Adapter, Index 2 (97% over 35bp)
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA178750.3482852771094056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG177430.34571332429382845TruSeq Adapter, Index 14 (97% over 43bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG171250.33367190883908093No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA129980.2532594143702408No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG128410.25020034927898616No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA119370.2325863693904881TruSeq Adapter, Index 14 (97% over 41bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA118190.23028719944929035No Hit
GGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTC112310.21883031872535577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG110040.21440733926220415Clontech Universal Primer Mix Long (95% over 21bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA107950.21033508063754033No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC106640.2077826123129903No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG104750.20410004350886846No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC101030.19685181284678743No Hit
GGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCC99780.19441625146839997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG94060.18327112260089898No Hit
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA90230.1758085625375198No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA89710.17479536900411066No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC88200.17185321085901858No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT86680.16889156822289947TruSeq Adapter, Index 14 (97% over 45bp)
ATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC79870.15562262983344463Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT78910.15375211869484304No Hit
AGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACT76570.14919274779450176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTG76160.14839388366239067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA74790.14572450839167803No Hit
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC73760.14371760581588675No Hit
GGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACT67270.13107217113929912Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC66920.13039021395335063No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA65290.12721424191593336No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG64910.1264738312569036Clontech Universal Primer Mix Long (95% over 22bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA64890.1264348622748494No Hit
TAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC62990.12273280897970046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA62140.12107662724239701Clontech Universal Primer Mix Long (95% over 24bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAA61920.12064796843980082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG61830.12047260802055691No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC59860.11663416328821828No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG59010.11497798155091483No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC56440.10997046735695022No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACAC56430.10995098286592313No Hit
GGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTC55950.10901572729662234Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG55900.10891830484148686Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC55410.10796356478115895No Hit
TCCTCGGCCGCGACCACGCTGCCCAGATCGGAAGAGCACACGTCTGAACT55040.10724263861315628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCAC54400.1059956311874219No Hit
ACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCT53320.10389130615649515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG52990.10324831795260085Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTAGTAT52720.10272223669486917TruSeq Adapter, Index 14 (97% over 44bp)
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG52480.10225460891021877No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC52330.10196234154481229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)