FastQCFastQC Report
Tue 1 Mar 2022
H3725BGXL_n01_NEN-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3725BGXL_n01_NEN-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891614
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA4181306.067228953914134Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA3372374.893440056277093Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA3283564.764573291539544Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2900654.208955986217452TruSeq Adapter, Index 13 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2155393.1275547353638786Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG832571.2080914572406405TruSeq Adapter, Index 2 (97% over 35bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGA686260.9957899557346073Clontech Universal Primer Mix Long (95% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAG435290.6316227229209297Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG401250.5822293587539871Clontech Universal Primer Mix Long (95% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCGGAAGA384330.557677780560548Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTG352920.5121006487014508No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA302330.4386925907341879Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG285260.4139233567057006TruSeq Adapter, Index 2 (97% over 35bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAG282950.41057145684595797No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA257700.37393272461284105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCG250010.362774235469369No Hit
GGTCGCGGCCGAGGATCATGACTCTTTCCCATTTAAATCCTTCCGGGAAG191220.2774676585194702No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGA172540.25036225186146527No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGA168850.24500791831927904No Hit
GGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTC168280.24418082614609582Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCA157060.227900169684489No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGC154580.22430159321169177No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATAGATCGGAAGAG153770.2231262517024314Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGCGGCCGAGGATCGTAGAAGTATTTCTAGTAATGGTGGTACACGTA150700.21867156227844448No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA145180.21066182754867TruSeq Adapter, Index 13 (97% over 40bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACAC141340.2050898381714356No Hit
GGTCGCGGCCGAGGATCAATCACCTCCAGCAGTGACACCAAGGTAGGGGT140390.2037113512161302No Hit
GGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCC139940.20305838371098556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG137440.1994307864601819TruSeq Adapter, Index 13 (97% over 39bp)
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACA123350.1789856483546525No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG123020.17850680551754639No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGC122360.17754911984333424No Hit
AGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACT111560.1618778997198624Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTCGCGGCCGAGGATCGAGATTGAAGGTGAAGGTGAGGGACGTCCATAC110300.16004959070545738No Hit
GGTCGCGGCCGAGGATCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATG109240.15851148947111662No Hit
GGTCGCGGCCGAGGATCAGAAAAACGCTACCTGCTCCTACGAAAGGTTCC109080.1582793232470652No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAGATCGGAAGAGCA106590.15466623638526475No Hit
CTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTG104390.15147395080455756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC99050.14372540307684092Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACT97350.14125863694629442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC94870.13766006047349721No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG92670.13446777489279Clontech Universal Primer Mix Long (95% over 22bp)
TAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC92080.13361166194160035Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACA88390.12825732839941414No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA88380.1282428180104109No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAA87110.12639999860700266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAG84160.12211943385105435No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAG82510.11972521966552392No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACACTCTCGTAT81210.11783886909510603TruSeq Adapter, Index 13 (97% over 44bp)
GGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTC80720.11712786003394851Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCGGCCGCGACCACGCTGCCCAGATCGGAAGAGCACACGTCTGAACT78320.113645366673177Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC77110.11188960960378802No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTAGATCGGAAGAGCACAC76910.11159940182372374No Hit
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG76880.1115558706567141Clontech Universal Primer Mix Long (95% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC76490.1109899654855887No Hit
ACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCT74440.10801533573992972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGAGATCGGAAGAGCACAC73090.10605643322449575No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCAGATCGGAAGAGCAC72860.10572269427742181No Hit
CCTCGGCCGCGACCACGCTGCCCAGATCGGAAGAGCACACGTCTGAACTC70760.10267551258674673Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT69870.10138408796546064TruSeq Adapter, Index 8 (97% over 36bp)
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTT69350.10062954773729348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)