FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31384495
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480940.7904986204175023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC72700.021.5152262
GTACTCT74200.020.8998783
CGGGAAC151050.020.2347321
TTGTACT78800.019.7240621
ATACGAG80200.019.1678035
TCTAGTT98150.017.8045887
GGAACGT155750.017.5608124
GAACGTA178050.016.8479824
CCGGGAA162400.016.6225221
TATTCAC207700.014.8725349
GGGAACG205300.014.3519953
GTATTCA206500.014.3046858
CCATTGT145300.014.108879145
GGTTAGA124250.012.5449564
TACTCTA138100.012.4048624
GTAGATA192150.012.327615145
ACGAGAT166900.012.3027957
CTAGTTG134000.011.5107448
GTTAGAG93150.011.2377825
TGGGCTA77650.011.0742522
Produced by FastQC (version 0.11.5)