FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_neg.11.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_neg.11.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10727590
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC251070.2340413830133329Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT223630.20846247852499955No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC145820.13592987800615047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTGT121050.046.7754029
TTGTACT129450.045.2625851
TCTAGTT132600.044.5009577
CCGTATA70700.043.07964145
TGTACTC137700.042.722322
CTAGTTG141850.040.73238
GTACTCT148750.040.0690963
TACTCTA155850.038.8849954
GGATATC52050.037.8600272
ACTCTAG178700.035.815655
CTCTAGT185900.034.0779766
ACGTGTT73750.033.82506145
GATATCT59900.033.6112483
GTAGATA254100.031.678202145
TCTCGGG58700.030.019976145
ATATCTT50650.030.0194844
GTACGTT45950.028.407248145
TATCTTG56000.026.116115
CGGTGGG76550.025.482925145
AGCATTA123650.024.8861754
Produced by FastQC (version 0.11.5)