FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_9109.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_9109.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17056602
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT351740.20621926923076475No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC270140.15837855629157555No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT247070.14485300178781213No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA225160.1320075358503411No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT211210.12382888455742826No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG191730.11240808690969044No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT178690.10476295337136904No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG176070.10322689126474312No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA172120.10091107243986816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT130950.052.33072145
CCGTATA27700.041.624374145
TATAGTT92700.032.541977145
TAGTGTT88750.032.029343145
TGTACTC32300.031.8512232
GAAACTA132150.031.7804744
CGCTAGG10900.031.2227294
AGTGTAT136150.030.785097145
GTAGATA206550.029.947058145
CTCGGCT60150.029.897241
TTGTACT33600.029.5662561
CGCTCAG47850.029.0548634
AACGTAT241100.027.7788836
CTAACCA150450.027.4336438
GAACGTA254800.026.3424195
GTATTCA261950.025.4042029
AGAAACT167550.025.2425293
AAGAAAC166300.025.1811662
TCGTGTT114000.025.125956145
CGTATTC270100.024.637228
Produced by FastQC (version 0.11.5)