FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_8404.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_8404.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20755662
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG180550.032.614403145
TCGTGTT242850.023.650372145
ATGTGTT197400.019.507349145
AGAGCGG201200.019.283094145
AACGTAT140250.014.1446666
CGAGTCT97050.013.4294628
GTGCCTG192250.013.164669145
ACGTATT153850.013.1299237
GAACGTA155950.013.0457835
CCGGGAA184300.012.5510051
GTAGATA173900.011.718112145
CTCTATT118450.011.571179145
ACCGAGT134500.011.30425556
AACCGAG172350.011.3003055
GGAACGT179750.011.2788964
TCTCGGG180500.010.847694145
CGTATTC193700.010.6909938
CGGAAGC250250.010.548196145
TGTCCTA88150.010.5132475
TATTCAC175350.010.282249
Produced by FastQC (version 0.11.5)