FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_8404.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_8404.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21596511
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG899610.41655339605550173No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC762490.35306165889480945No Hit
CCGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAACGCCTCCA458910.21249265679998036No Hit
CCGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAACGTCTCCT435040.20143994555416844No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG281450.1303219765451929No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC266430.12336714944372264No Hit
CTGGGTTAGAGTAGCCATAACACAAGGGTAGTATCCCAACAACGCCTCAC263310.12192247164368356No Hit
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG228810.10594766904709746No Hit
GCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCAGT222160.10286846796688594No Hit
CTGGGCTACACACGTGCTACAATGGCTGGTACAACGAGTCGCAAGCCGGT218810.10131729148286962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA282550.051.929382
GTTAGAG400950.047.218895
TTAGAGT410550.046.3080986
CATTGTA347900.045.105614145
GGTTAGA480400.044.7756584
CCGGGTT354850.041.446531
CCGGGAA484350.039.8428731
TAGAGTG415600.037.0188337
GGGTTAG589300.036.511633
GAGTGGC434800.034.804099
TTTCCGT345200.032.896427145
CCATTGT509300.032.548447145
CGGGAAC676150.032.4878731
AACGTAT707000.032.267355
GGGAACG708550.031.6602152
GAACGTA749650.030.6252774
ACGTATT750550.030.5103956
TATTCAC745800.030.416669
GGAACGT750400.029.8987923
TGGGCTA381800.029.8901482
Produced by FastQC (version 0.11.5)