FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_8336.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_8336.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22324661
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT213600.061.8561145
CCGTATA53600.048.028492145
AGTGTAT211150.034.54954145
GTACTCT148200.034.2051243
TAGTGTT130850.033.25163145
TTGTACT159650.031.5646211
GTAGATA346550.030.676332145
TAGTTGT164550.030.5369789
TGTACTC169950.030.0118242
TATAGTT192450.028.863317145
TCTAGTT183900.028.6996887
TACTCTA183900.028.5428524
TCGTGTT171550.022.065569145
AACGTAT167100.019.410076
GAACGTA179600.018.2613544
CACCTTA121450.018.2420988
CGTATTC182400.017.8623478
CTAGTTG307150.016.311978
CGGGAAC215500.016.3055462
CGGTGGG117750.015.950498145
Produced by FastQC (version 0.11.5)