FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_7784.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_7784.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757759
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG431880.2577194241783761No Hit
CTGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAGCGTCTCCT418860.24994988888430728No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG414780.24751519579676493No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC407440.24313513519319618No Hit
CGGAAAGACCCCATGGAGCTTTACTGCAGTTTGATATTGAGTGTCTGTAC340130.2029686666337665No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC322120.19222140621547307No Hit
GGGAAATCTCATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTT299120.17849642067295515No Hit
CTGGGCTACACACGTGCTACAATGGCTGGTACAACGAGTCGCAAGCCGGT263390.15717495400190443No Hit
CTGGAACGCATAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTG261710.15617243331879876No Hit
ATGCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCA233560.1393742444917605No Hit
GCCCGTCAGACACTGTCTCCGATAGGGATCACCTATCTGGGTTAGAGTGG230030.13726775758023493No Hit
GCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCAGT218890.13062009066964145No Hit
CTCGCAGTCAAGCTCCCTTATACCTTTACACTCTGCGAATGATTTCCAAC186720.11142301306517179No Hit
GTCAGACACTGTCTCCGATAGGGATCACCTATCTGGGTTAGAGTGGCCAT171150.10213179459138899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCATC119750.042.2621577
CATTGTA181450.039.165417145
CCGTATA17500.035.222042145
CGCGAGC155250.034.8881951
GACATAC32800.033.9961179
GGATGAC232550.030.884385
TGGGGAT231450.030.8003982
GATGACG234300.030.406046
GGGGATG242350.030.2097843
CGGATGT23300.030.1859821
CTCATCT169900.029.235078
GAAATCT188150.029.0591013
GGGAAAT195200.028.6022661
CCATTGT275550.027.869497145
TAAGTTA49750.027.8136942
ATCTCAT191350.027.6583296
ATGACGT255900.027.5574287
AAATCTC197300.027.4865554
GGGATGA275500.026.7016244
TCGTGTT94600.026.369444145
Produced by FastQC (version 0.11.5)