FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_7784.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_7784.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19885735
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT380940.19156445562610586No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC329130.16551060345519036No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT274220.13789784486215872No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC226170.11373479531935832No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT218160.10970678227382594No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG210810.10601066543429248No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA203080.10212345683978993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT63100.034.0126081
GATCCTG69350.033.6144265
GAAACTA121900.033.4977534
AACGTAT413550.032.701315
GTATTCA432700.031.2226248
TCGATCT25100.030.0038833
GAACGTA467200.029.7535444
TGGGCTA77800.029.4257472
CGTATTC459700.029.3401477
TATTCAC461900.029.312559
TCCCTGA105700.029.2530233
CGGGAAC368850.029.0144371
AAGAAAC141800.028.662012
ACGTATT479950.028.6143786
GATCATG46850.028.2785915
TTGAACG75700.028.213057
GGAACGT395350.027.5976334
GGCGAAT51200.026.906751
TCAGATT83250.026.8031882
CTCCCTG118250.026.4056052