FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_6852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_6852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35298285
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA500700.024.634091
GAACGTA556550.023.9344065
CGGGAAC516750.023.9325452
AACGTAT565700.023.5213136
GGAACGT554400.022.9705474
CGTATTC593450.022.4106758
ACGTATT629600.021.1919467
CATTGTA382000.020.996243145
TCGTGTT248400.020.231659145
GTATTCA690900.019.3759379
GGGAACG707650.018.8806133
ACCGAGG131900.018.8806046
GATCATG215650.018.4638485
CCATTGT517000.018.333057145
TATTCAC679400.018.3079039
TTGAACG276350.017.4733667
GAGCTCG152200.017.3147668
TAACCAG330450.017.089529
GTGCGCG103850.016.8012078
CCAACCG147900.016.5006083
Produced by FastQC (version 0.11.5)